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DEVELOPMENT OF MICROSATELLITE MARKERS FOR AULONEMIA ARISTULATA (POACEAE) AND CROSS-AMPLIFICATION IN OTHER BAMBOO SPECIES

机译:竹节虫微卫星标记的开发和其他竹种的交叉扩增

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摘要

Premise of the study: Microsatellite primers were developed for Aulonemia aristulata, an endangered species of economic interest, to further describe its genetic variability and population structure. We also tested cross-amplification in 18 other bamboo species. Methods and Results: Using an enrichment genomic library, 13 microsatellite loci were isolated and characterized in A. aristulata. Seven of these loci were polymorphic. Twelve markers were cross-amplified in at least ten of the tested bamboo species. Conclusions: These markers will be useful for studies on the genetic diversity and structure of A. aristulata, which are important for future conservation, management and breeding programs of this species.
机译:研究的前提:已开发了一种微卫星引物,用于研究一种濒临灭绝的具有经济价值的物种Aulonemia aristulata,以进一步描述其遗传变异性和种群结构。我们还测试了其他18种竹子的交叉扩增。方法和结果:利用丰富的基因组文库,分离了13个微卫星基因座并在拟南芥中进行了鉴定。这些基因座中的七个是多态的。在至少十个被测竹种中,十二个标记被交叉扩增。结论:这些标记物将有助于研究A. aristulata的遗传多样性和结构,这对于今后对该物种的保存,管理和育种计划都具有重要意义。

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